NO2-CLA binding to HSA.
A, UV-Visible spectra of 16 μm
thiol-blocked delipidated HSA (gray trace), 10
μm NO2-CLA (black
trace), and the combination of both reagents
(dashed trace). B,
thiol-blocked delipidated HSA (0.2–10 μm)
was mixed with NO2-CLA (10 μm), and
UV-visible spectra were recorded. The absorbance at 310 nm was
plotted against HSA concentration, and the amount of
NO2-CLA bound was determined from the change in
the slope (open circles). A control without
NO2-CLA was included (black
circles).