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. 2016 Dec 6;292(4):1145–1159. doi: 10.1074/jbc.M116.756288

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FIGURE 9. — NO₂-CLA binding to HSA. A, UV-Visible spectra of 16 μm thiol-blocked delipidated HSA (gray trace), 10 μm NO₂-CLA (black trace), and the combination of both reagents (dashed trace). B, thiol-blocked delipidated HSA (0.2–10 μm) was mixed with NO₂-CLA (10 μm), and UV-visible spectra were recorded. The absorbance at 310 nm was plotted against HSA concentration, and the amount of NO₂-CLA bound was determined from the change in the slope (open circles). A control without NO₂-CLA was included (black circles).