FIGURE 5.
cAMP has no effect on acetylation promotion of mutant SeAcs. A, cAMP activates SePat-dependent acetylation of Lys609 on wild-type SeAcs but has no effect on mutant SeAcs in vitro. B, cAMP inhibits SeCobB-dependent deacetylation of Lys609 on wild-type SeAcs but has no effect on mutant SeAcs in vitro. Top band, acetylation detection by Western blotting using anti-SeAcs Lys609ac antibody; bottom band, Coomassie Brilliant Blue-stained proteins on SDS-PAGE gels are shown as a loading control. Each experiment was independently repeated three times.