FIGURE 2.
Wdr1 KO embryos in peri-implantation development. A, after mating with Wdr1f/− males, oocyte specific knock-out females were injected with Chicago blue. Then, the uteruses were picked up at E5.5 and imaged by differential interference contrast. Scale bar, 5 mm. B, deciduas with embryos from A were frozen and sectioned (8 μm). The sections were stained by H&E and imaged. Two types such as (a) and (b) were observed. The percentage of the two types was counted from three pregnant females. Statistical results are represented as mean ± S.D. Scale bar, 50 μm. C, oocyte-specific Wdr1 KO females were mated with Wdr1f/− males. At E3.5, blastocysts were flushed from the uterus and imaged by differential interference contrast. Scale bar, 40 μm. D, blastocysts obtained from C were cultured in vitro for 3 days and treated with EdU for 2 h before being fixed. The outgrowth was stained for EdU (red) according to the protocol provided by the manufacturer. Nuclear DNA was stained by Hoechst 33342 (blue). Scale bar, 200 μm. E, Wdr1f/− (control, CTL) and Wdr1−/− (knock-out, KO) embryos were genotyped with specific primers.