FIGURE 5.

Deep sequencing analysis of separately sorted double NNK libraries. A, heat map of robust enrichment ratios (see under “Materials and Methods”) of double amino acid substitutions of the original cetuximab meditope. All RERs above 1 are indicated in the respective squares. At each position, the original residue is encircled. B, cetuximab titration to 10 nm FITC-conjugated synthetic peptides in PBS, pH 7.4 1 mg ml⁻¹. Fluorescence polarization was used as a readout. Data points represent mean ± S.D. of triplicate measurements. The indicated mutations are with respect to Md1. For exact peptide sequences, see supplemental Table S2 peptides 13–16.