TABLE 1.

Dissociation constants of mutant peptides

Sequencea	Kd b
	nm
Meditope 1	129.4 ± 4.7
Q1V	111.5 ± 3.6
S5G	113.9 ± 3.1
K10R	81.3 ± 1.9
Q1V/S5G	69.4 ± 2.2
Q1V/K10R	63.2 ± 1.2
S5G/K10R	41.1 ± 1.2
Q1V/S5G/K10R	30.3 ± 0.9

^a Exact peptide sequences (including labels, linkers, and flanking residues) are in supplemental Table S2, numbers 5–12.

^b K_d values were calculated with fluorescence polarization measurements (Fig. 3D). Values are mean ± S.D. from the fit of Hill curves through the data presented in Fig. 3C.