FIGURE 6.

Tunicamycin reduces the LDL cholesterol content of plasma. 12-Week-old C57BL/6 female mice were subcutaneously injected with a single dose of TM (500 μg/kg) and sacrificed 24 h later. A and B, plasma was collected after sacrifice and total cholesterol and triglycerides were quantified using enzymatic assays. A and B, *, p < 0.05 versus vehicle. C, to determine the effect of TM on LDL cholesterol, plasma apoB48 and apoB100 were examined using immunoblots. Immunoblot analysis was also used to ensure TM-mediated induction of UPR markers GRP94, GRP78, and IRE1α. D, to further elucidate the effect of TM on all lipoprotein fractions, FPLC analysis was used to construct a lipid profile. Differences between treatments were assessed with paired Student's t tests, and all values are represented as mean ± S.D. E, *, p < 0.05 versus vehicle (NT). Veh, vehicle.