Figure 6.

The r1–7 region is required for eIF4B binding to eIF4A. Supernatant depletion assay with eIF4A-bio and 0.5 µM of either eIF4B_ΔCTD (A), eIF4B_ΔNTDΔRRM (B) or eIF4B_Δr1–7 (C) in the presence of 5 µM 32mer RNA and 10 mM ADPNP (Coomassie Blue staining). eIF4B in the supernatant was quantified by densitometry. Red: depletion upon addition of streptavidin beads (negative control), yellow: depletion upon addition of eIF4A-conjugated streptavidin beads. The quantification shows the mean values from 2 independent experiments with standard errors.