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. 2016 Nov 18;16(1):59–72. doi: 10.1080/15384101.2016.1252881

Figure 3.

Figure 3.

BODIPY staining in SH-SY5Y during differentiation (A). Undifferentiated (CTR) and differentianted (N2) cells at 4h and 24h from N2 treatment. Nuclei were counterstained with DAPI. Bar = 10 μm. B: BODIPY staining quantification expressed as Signal Intensity/Unit Surface Area. C: WB and relative densitometric analysis for Plin2 in undifferentiated (CTR) and differentiating (N2) cells at the indicated time-points. The relative densities of the immunoreactive bands were determined and normalized with respect to GAPDH, using a semiquantitative densitometric analysis. Data are mean ± SE of 4 different experiments. **P ≤ 0.005 and ***P ≤ 0.0005.