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. 2016 Nov 1;14(1):73–89. doi: 10.1080/15476286.2016.1251542

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© 2017 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 5. — BicD is needed for apical mRNA localization in follicle cells. 6.5% of the mRNA tested show apical localization in follicle cells. This mRNAs class is enriched 8-fold among the Egl::GFP targets compared to a random set (6.5% vs 0.8%¹¹) (A) In situ hybridization to wild-type (OreR, wt) controls and to egg chambers 4 d after turning off BicD expression (BicD^mom). Oocyte enrichment (arrows) as well as apical follicle cell localization of CG33129, egl and Uba1 was impaired when BicD was off. High magnification of the stage 10 follicle cell epithelium is shown in the right panels. Note that apical is toward the oocyte (up in the magnified pictures). Scale bars are 20 µm. Uba1 apical enrichment is weaker and less cortical, but still requires BicD. Histograms depicted on the right side of the pictures show the fluorescence intensity along the apical-basal axis of the marked cortical region around the nuclear layer (dotted square). This region is also shown magnified on the right side of the micrographs. (B) BicD mRNA expression (red) is drastically reduced in stage 9–10 BicD^mom egg chambers in the germline and also in the somatic follicle cells (high power pictures on the right). Note that BicD mRNA is also apically localized as expected since it is also a target of Egl. (C) BicD protein (red) is also apically enriched in wild-type follicle cells, but is almost undetectable in BicD^mom egg chambers, oocytes and follicle cells. Hoechst (blue) visualizes the DNA. Scale bars are 20 µm.