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MazF-mt9 cleavage of tRNA is sequence- and structure-dependent. A yellow arrow indicates that cleavage occurs at the position shown. Wild type sequence at the anticodon, red; mutated sequences, blue. Mutants with a red X over the structure were not cleaved by MazF-mt9. Full-length tRNA colored as in Fig. 2. Numbering in the anticodon indicates mature tRNA nucleotide position.
