Table 1.
Compilation of MazF RNA cleavage recognition sequences and targets.
| Toxin name | Alias | Organism | RNA target | Cleavage specificity | # of sites (mut) | Reference(s) | Other reported consensus | # of sites (mut) | Alternate reference(s) |
|---|---|---|---|---|---|---|---|---|---|
| MazF | ChpAK | Escherichia coli | 16S rRNA, mRNA | ↓ACA or A↓CA | 47 (16) | Zhang et al. 2003 & 200519,20; Vesper et al. 201127; Miyamoto et al. 2016a39 | A↓AC or U↓AC | 12 (0) | Munoz-Gomez et al., 200440 |
| MazF-mt1 | MazF9 | Mycobacterium tuberculosis | mRNA | U↓AC | 2 (5) | Zhu et al. 200626 | None | N/A | N/A |
| MazF-mt3 | MazF6 | M. tuberculosis | 23S/16S rRNA, mRNA | U↓CCUU | 273 (0) | Schifano et al. 201324 | UU↓CCU or CU↓CCU | 12 (0) | Zhu et al. 200822 |
| MazF-mt6 | MazF3 | M. tuberculosis | 23S rRNA, mRNA | UU↓CCU | 21 (0) | Schifano et al. 201425 | U-rich or YU↓WCY | 3 (0) | Zhu et al. 200626 |
| MazF-mt7 | MazF4 | M. tuberculosis | mRNA | U↓CGCU | 23 (0) | Zhu et al. 200822 | None | N/A | N/A |
| MazF-mt9 | MazF7 | M. tuberculosis | tRNA, (mRNA) | UU↓U + stem | 220 (0) | Schifano et al. 201623 | None | N/A | N/A |
| MazF-hw | HQ2202A | Haloquadra walsbyi | mRNA | UU↓ACUCA | 5 (8) | Yamaguchi et al. 201217 | None | N/A | N/A |
| MazF-bs | EndoA, YdcE | Bacillus subtilis | mRNA | U↓ACAU | 12 (4) | Park et al. 201141 | ↓UAC or U↓AC | 1 (5) | Pellegrini et al. 200542 |
| MazF-cd | CD3461 | Clostridium difficile | mRNA | U↓ACAU | 11 (0) | Rothenbacher et al. 201216 | None | N/A | N/A |
| MazF-sa | SA2058 | Staphylococcus aureus | mRNA | U↓ACAU | 9 (3) | Zhu et al. 200921 | V↓UUV' | 7 (12) | Fu et al. 200743 |
| MazF-seq | None | Staphylococcus equorum | mRNA | U↓ACAU | 9 (4) | Schuster et al. 201344 | None | N/A | N/A |
| MazF-ne | NE1181 | Nitrosomonas europaea | mRNA | A↓AU | 25 (7) | Miyamoto et al. 2016b45 | None | N/A | N/A |
| MazF-pp | PP0771 | Pseudomonas putida | mRNA | U↓AC | 25 (7) | Miyamoto et al. 2016a39 | None | N/A | N/A |
The number of MazF-mediated cleavage sites used in each reference to determine a consensus cleavage recognition sequence is indicated, along with the number of mutants (“mut,” in parentheses) that were tested in cleavage assays. The total cleavage sites and the tested mutants listed for E. coli MazF were combined from the _indicated 4 references. mRNA in parentheses for MazF-mt9 indicates that this class of RNA is not the primary target of this toxin Y = C, U; W = A, U; V = A, C, G.