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. 2016 Nov 14;16(1):73–81. doi: 10.1080/15384101.2016.1252882

Figure 2.

Figure 2.

Expression changes of metabolism related proteins were measured by Western blot and enzyme activity assay kits. (A) The expression of proteins involved in KREBS cycle and glycolytic pathway in colorectal cancer cells. The two neighboring columns represent expression of indicated proteins in cancer cell lines co-cultured with CCD-18-co compared to their control groups, respectively. β-actin served as control for normalization. *P< 0.05 vs. corresponding negative respectively, **P < 0.01 versus corresponding negative respectively. (B) The expression of proteins involved in KREBS cycle and glycolytic pathway in CCD-18-co cultured with cancer cells or not. β-actin served as control for normalization. *P < 0.05 vs. negative respectively, **P < 0.01 versus negative respectively. (2C&2D) Analysis of PKM2 and IDH2 activities in 3 tumor cell lines. *P < 0.05 vs. negative respectively, **P < 0.01 versus negative respectively. (2E&2F) Analysis of PKM2 and IDH2 in CCD-18-co cultured alone or co-cultured with tumor cells. *P < 0.01 vs. negative respectively.