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. 2016 Nov 14;16(1):73–81. doi: 10.1080/15384101.2016.1252882

Figure 3.

Figure 3.

Expression of oxidative stress and autophagy related proteins in fibroblasts co-cultured with colorectal cancer cells were measured by western blot and immunofluorescence. (A) Expression of oxidative stress related proteins SOD2 and GSTP1 in CCD-18-co cells co-cultured with tumor cells, β-actin served as loading control. *P < 0.05 versus negative respectively, **P < 0.01 vs. negative respectively. (B) Immunofluorescence observation of SOD2 (red) in CCD-18-co cells co-cultured with tumor cells (63×). (C) Expression of autophagy related proteins LC3, BNIP3 and p62 in CCDs co-cultured with tumor cells. **P < 0.01 versus negative respectively. (D) Immunofluorescence observation of LC3 (green) in CCDs co-cultured with tumor cells (63×).