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Production of hHAP stem-cell colonies. Human hair follicle culture protocol: isolated upper parts of human hair follicles were cultured in DMEM containing 10% FBS. Four weeks after culture growing cells from the upper parts of human hair follicle were transferred to DMEM/F12 without FBS. One week after culture, the growing cells formed many hHAP stem-cell colonies. Two days after transfer to DMEM containing 10% FBS, hHAP stem-cell colonies started to differentiate.
