Table 2.
Pr55Gag binding to the first 600/615 nucleotides of genomic and mutant RNAs: the impact of each SL of the Psi region. Binding parameters were derived from the Scatchard model.111 Kdi (i = 1, 2, 3) corresponds to the different classes of Pr55Gag binding sites from the highest to lowest estimated affinity. Stoichiometry analysis revealed the number of bound Pr55Gag to the 3 different classes of binding sites. RNAs derived from NL4.3 isolate have a name starting with “N.” Those derived from MAL isolate have a name starting with “M.”a Mean ± SD of at least 3 independent experiments.
| Stoichiometry |
||||||
|---|---|---|---|---|---|---|
| RNA | Kd1 (nM)a | Kd2 (nM)a | Kd3 (nM)a | site 1 | site 2 | site 3 |
| N1-600WT | 1.8 ± 0.4 | 14.6 ± 3.8 | 6.9 ± 0.7 | 3.1 ± 0.8 | ||
| M1-615WT | 2.9 ± 0.6 | 15.4 ± 3.4 | 6.7 ± 1.1 | 3.0 ± 0.9 | ||
| M1-615 ΔSL1 | 21 ± 2 | 3.2 ± 0.2 | ||||
| N1-600 ΔSL1 | 16 ± 3 | 3.2 ± 0.1 | ||||
| M1-615SL1sAL | 12.5 ± 2.7 | 65.5 ± 6.6 | 1.9 ± 0.2 | 1.1 ± 0.2 | ||
| N1-600SL1sAL | 9.5 ± 2.4 | 62.5 ± 5.8 | 2.1 ± 0.4 | 0.97 ± 0.3 | ||
| N1-600 ΔSL2 | 6 ± 2 | 55.7 ± 11.8 | 5.0 ± 0.7 | 2.2 ± 0.5 | ||
| M1-615 ΔSL3 | 3 ± 1 | 40.5 ± 6.5 | 3.2 ± 0.3 | 1.6 ± 0.2 | ||
| M1-615 ΔSL4 | 4 ± 1 | 58.5 ± 7.5 | 3.1 ± 0.6 | 1.6 ± 0.1 | ||