Table 3.

Impact of deletion/mutations of the internal loop in SL1 on Pr55^Gag binding. Binding parameters were derived from the Scatchard model.¹¹¹ For long RNA fragments we detected 3 classes of binding sites, K_di (i = 1, 2, 3) corresponds to the different classes of Pr55^Gag binding sites from the highest to lowest estimated affinity. Stoichiometry analysis revealed the number of Pr55^Gag bound to the different classes of binding sites.^a Mean ± SD of at least 3 independent experiments. n.d.: not determined.

RNA		Kd (nM)a	Pr55Gag/RNA
N35SL1		5 ± 2	3.50 ± 0.65
N35SL1 ΔIL		18 ± 1	2.1 ± 0.4
					Stoichiometry
RNA	Kd1 (nM)a	Kd2 (nM)a	Kd3 (nM)a	Site1	Site2	Site3
N1-600WT	1.8 ± 0.4	14.6 ± 3.8		6.9 ± 0.7	3.1 ± 0.8
N1-600SL1srIL			53.6 ± 9.1			2.4 ± 0.2
N1-600SL1syIL	n.d.	n.d.	n.d.
N1-600SL1 ΔIL	n.d.	n.d.	n.d.