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. Author manuscript; available in PMC: 2018 Jan 19.
Published in final edited form as: Mol Cell. 2017 Jan 19;65(2):207–219. doi: 10.1016/j.molcel.2016.12.008

Figure 3. mRNA structure downstream of SS promotes efficient -1 PRF in the copA gene.

Figure 3

(A) The predicted structure of the pseudoknot in the copA mRNA downstream from the SS. The slippery site is shaded in gray, the -1 frame stop codon is boxed, and stems S1 and S2 of the pseudoknot are indicated.

(B) Predicted secondary structure of the mRNA segments downstream of the SS in the 3′-truncated copA templates. The boundaries of the CopA(Z) coding segment is indicated and the location of SS is marked by an arrow.

(C) SDS-gel analysis of the [35S]-labeled protein products expressed in vitro from the 3′-truncated copA templates shown in (B). Grey arrowheads indicate the bands corresponding to the full-sized 0 frame product; black arrowheads indicate CopA(Z) generated via -1 PRF.

(D) Quantification of the -1 PRF efficiency in the different 3′-truncated copA templates from the relative intensity of the bands indicated in (C). Error bars show standard deviation from the mean in 3 independent experiments.

See also Figure S3.