Table 1. Comparison of summerized apoptotic features in rat granulosa cells from DES- and eCG-primed ovaries cultured with IFN-γ and Fas mAb.
Origin of cells | Treatment | Cell floating(phase contrast) | Membrane blebbing(phase contrast) | TUNEL |
---|---|---|---|---|
DES-primed | IgG | - | - | - |
IFN-γ | - | - | + | |
Fas mAb | +++ | ++ | +++ | |
IFN-γ+Fas mAb | ++++ | ++++ | ++++ | |
eCG -primed | IgG | - | - | - |
IFN-γ | - | - | - | |
Fas mAb | ++ | ++ | ++ | |
IFN-γ+Fas mAb | +++ | ++ | +++ |
-: rare, +: occasional, ++: frequent, +++: extensive, ++++: very extensive