Figure 3.

Differential splicing of PPFIBP1 transcripts in the CE. Sashimi plot showing the number of reads spanning individual splice junctions are shown next to lines connecting the exons involved. Alternative Ensembl transcript structures are shown at the bottom of the figure in blue. (B) RT-PCR using primers that flank selected exons was used to assess exon inclusion from PPFIBP1 in corneal endothelial RNA samples from controls (lanes 1–3) and FECD patients (lanes 5–8). The repeat expansion status of each sample is shown (+, >45 repeats; ≤45 repeats). Lane 4 contains DNA size markers.