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. 2016 Nov 18;12(1):41–54. doi: 10.1080/15592294.2016.1260211

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© 2017 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 7. — Single molecule methylation analysis in ESC neural differentiation model. A) Averaged methylation at Ddo R4 region in undifferentiated ESCs (U-ES) and in differentiated ESCs (nD-ES) from 3 independent experiments. B) Averaged methylation of each CpG site (−363, −330, −318, −242, −175, and −125) at Ddo R4 region (data are showed as mean ± standard deviation; **P < 0.01). C) Microscope images of ESCs in undifferentiated state (U-ES) and upon differentiation toward neuronal phenotype (nD-ES). Bar = 200 µm. Epiallele frequency distribution in 3 independent ESCs differentiation experiments (I, II, and III). The pie charts represent the relative percentage of fully unmethylated epialleles (U = white), fully methylated epialleles (M = black), and the 62 intermediate epialleles (I = gray gradient). The line plots represent the frequency distribution of each of the 62 intermediate epialleles (blue: undifferentiated ESC; red: differentiated ESCs). D) Comparison between average frequency distribution of each of the 62 Ddo intermediate epialleles, from 3 independent experiments, in undifferentiated (blue line) vs. differentiated (red line) ESCs. The specific methyl CpG combinations of each of the 62 intermediate epialleles are indicated on the x-axis.