Figure 4. Effect of S. haenkei treatment on photo ageing of human fibroblasts.

WICCL75 human fibroblasts were irradiated with 30J/m² UVB and 3h later treated with 10μg/ml S. haenkei extract. (a) Proliferation of irradiated human WI38 fibroblasts treated with S. haenkei extract. Cell proliferation was measured by Crystal violet staining at time points treatment (10μg/ml) 24h and 48h and represented as fold change in growth (compared to untreated control). Results are expressed as mean values (+SEM) for duplicate in each group in one representative experiment out of three independent experiments. (b) Senescence of irradiated human WI38 fibroblasts treated with S. haenkei extract. The graph represents percentage of β-galactosidase positive cells revealed in culture at time points 24h and 48h. Quantifications were done on 4 images (roughly 500 cells) per experiment by determining the ratio of perinuclear blue–positive to perinuclear blue–negative cells. Results are expressed as mean values (+SEM) of cell count in three independent experiments.