Table 1. Senescence induction of immortal cell lines by NonO.
To assess the generality of NonO overexpression-induced senescence, population doublings (PDs) were determined for the indicated cell lines following stable transfection with PCR3.1-NonO; PCR3.1, mock, vector control.
| Cell line | DNA transfected | No of clones lost proliferation/total number of clones | PD achieved prior to loss of proliferation |
|---|---|---|---|
| Cos7 | pCR3.1-NonO | 16/36 | 8-96 |
| pCR3.1 | 0/16 | ND | |
| CHO | pCR3.1-NonO | 0/36 | ND |
| pCR3.1 | 0/16 | ND | |
| NIH3T3 | pCR3.1-NonO | 0/36 | ND |
| pCR3.1 | 0/16 | ND | |
| Cos7 | pCR3.1-NonOAS | 17/36 | 4-76 |
| pCR3.1-PSF | 11/36 | 16-96 | |
| pCR3.1-PSFAS | 8/36 | 16-52 |