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. 2016 Nov 17;10(1):151–161. doi: 10.1111/1751-7915.12433

Figure 7.

Figure 7

Conversion rates of TS in growing‐cell processes. The strains Mycobacterium smegmatis MS6039‐5941 (pHSDCT) (red bars) and M. smegmatis MS6039‐5941 (pHSDCL) (blue bars) were grown at minimal medium containing 1.8 mM cholesterol (substrate) and 18 mM glycerol (carbon and energy source). The following culture conditions were tested: (A) Without addition of any carbon source; (B) with addition of 1% glucose at 24 h; (C) with addition of 1% glucose at 69 h; (D) with addition of 1% glycerol at 69 h; (E) without addition and 1% glucose instead of glycerol as initial substrate. The molar conversion rate of TS (first and second bars) and the ratio of TS to androstenes (third and fourth bars) at the end of the culture (93 h) are shown. The molar conversion rate was calculated on the basis of 1.8 mM cholesterol added. Androstenes are calculated by adding AD and TS. Average and standard deviation of two biological replicates are represented.