Skip to main content
. Author manuscript; available in PMC: 2017 Jan 27.
Published in final edited form as: Methods Mol Biol. 2016;1478:53–78. doi: 10.1007/978-1-4939-6371-3_3

Table 1.

Available Q-system reagents

Stock number Q-transgene Purpose/function Reference
Driver lines with original QF
36347 iav-QF Expresses QF in chordotonal organs in the pattern of the iav (inactive) gene [74]
36346, 36349 nompC-QF Expresses QF in chordotonal organs vchA, vchB, lch5 and lch1, the class III sensory neurons vdaD, v’pda, ldaB, ddaA and ddaF and the dmd1 sensory neurons in the pattern of the nompC gene. [74]
36357 Synj-QF Expresses QF in the nervous system, imaginal disks, gut and salivary glands in the pattern of the synj (Synaptojanin) gene. [74]
36365 Tbh-QF Expresses QF in the larval nerve cord in the pattern of the Tyramine beta-hydroxylase gene, targets octopaminergic neurons. [75]
52244, 52245 Tdc2-QF Expresses QF under control of Tdc2 (Tyrosine decarboxylase 2) regulatory sequences, targets tyraminergic neurons. [76]
52250, 52251, 57359, 57362 Trh-QF Expresses QF under control of Trh (Tryptophan hydroxylase) regulatory sequences. [76]
36345, 36348 TrpAl-QF Expresses QF in class IV larval sensory neurons in the pattern of the TrpAl gene. [74]
36370 Trpl-QF Expresses QF in Bolwig’s organ and eye imaginal disks in the pattern of the trpl gene. [74]
30014, 30015, 30037, 30038, 30039 GH146-QF Expresses QF in subsets of projection neurons. [15]
41573 Mz19-QF Expresses QF in olfactory projection neurons. [51]
30016, 30043, 30042, 30019, 30018 EnhancerTrap-QF Enhancer trap lines generated by random insertion at various genomic loci [15]
30017, 30020 SwappableEnhancer Trap-QF Enhancer trap lines generated by random insertion at various genomic loci. These lines allow replacement of QF with another gene. [15]
57361, 57363, 57364 Ddc-FRT-stop-FRT-QF Expresses QF in the pattern of the Ddc (Dopa decarboxylase) gene upon FLP-mediated removal of a transcriptional stop cassette. Contributed by Tom Hartl and Matt Scott
57358, 57365 Trh-FRT-stop-FRT-QF Expresses QF in the pattern of the Trh (Tryptophan hydroxylase) gene upon FLP-mediated removal of a transcriptional stop cassette. Contributed by Tom Hartl and Matt Scott
InSITE-QF Enhancer-trap lines from InSITE collection [64]
IR76b-QF Expresses QF in a subset of neurons in the pattern of chemosensory receptor IR76b [77]
Or67d-QF Expresses QF in a subset of neurons in the pattern of olfactory receptor OR67d [59]
Driver lines with QF2
51957, 51958 αTubulin-QF2 Expresses QF2 ubiquitously under the control of αTubulin84B [41]
51955, 51956, 51959 nsyb-QF2 Expresses QF2 pan-neuronally under the control of n-syb. [41]
60320 Chat-QF2 Trojan-MiMIC line that expresses QF2 in the pattern of cholineacetyl transferase, targets cholinergic neurons. [66]
60315 vGlut-QF2 Trojan-MiMIC line that expresses QF2 in the pattern of vesicular glutamate transporter, targets glutamatergic neurons [66]
60323 Gad1-QF2 Trojan-MiMIC line that expresses QF2 in the pattern of glutamic acid decarboxylase 1, targets GABAergic neurons [66]
Driver lines with QF2w(eak)
51961 Actin5C-QF2w Expresses QF2w under the control of the Act5C promoter. [41]
51962, 51963 αTubulin-QF2w Expresses QF2w ubiquitously under the control of αTubulin84B [41]
51960, 51964, 51965 nsyb-QF2w Expresses QF2w pan-neuronally under the control of n-syb. [41]
59283 pGMR-QF2w Expresses QF2w under the control of the GMR promoter in the eye-antennal imaginal disk. [41]
Driver lines with GAL4QF
51945, 51946, 51947 nsyb-GAL4QF Expresses the GAL4 DNA-binding domain fused to the QF2w activation domain pan-neuronally under the control of n-syb. [41]
59284, 59285 Actin5C-GAL4QF Expresses GAL4QF under the control of the Act5C promoter. [41]
Driver lines with LexAQF
51953, 51954 nsyb-LexAQF Expresses the LexA DNA-binding domain fused to the QF activation domain pan-neuronally under the control of n-syb. [41]
62567, 62568 Actin5C-LexAQFw Expresses LexAQFw under the control of the Act5C promoter. [41]
60319 Chat-LexAQF Trojan-MiMIC line that expresses LexAQF in the pattern of cholineacetyl transferase, targets cholinergic neurons. [66]
60314 vGlut-LexAQF Trojan-MiMIC line that expresses LexAQF in the pattern of vesicular glutamate transporter, targets glutamatergic neurons [66]
60324 Gad1-LexAQF Trojan-MiMIC line that expresses LexAQF in the pattern of glutamic acid decarboxylase 1, targets GABAergic neurons [66]
Available by request from C. Potter, JHU αTubulin-FRT-stop-FRT-LexAQF Expresses LexAQF ubiquitously under the control of αTubulin84B upon FLP-mediated removal of transcriptional stop cassette
QUAS reporter lines
52259, 52260 10xQUAS-Chr2.T159C-HA A variant of Channelrhodopsin, excitatory blue light-activated sodium channel, tagged with HA. [76]
58401, 58402 QUAS-FRT-stop-FRT-ChR2.TR-mCherry A variant of Channelrhodopsin, excitatory blue light-activated sodium channel tagged with mCherry, that will be expressed upon excision of FRT-stop-FRT cassette. [59]
58400 QUAS-ChR2.TR-mCherry A variant of Channelrhodopsin, excitatory blue light-activated sodium channel, tagged with mCherry. [59]
36356, 36367 QUAS-CHETA.YFP A variant of Channelrhodopsin, excitatory blue light-activated sodium channel, tagged with YFP. [74]
55106, 55107, 55108, 55109 55110, 55115, 55116, 55120 QUAS-X∷CycB Cyclin B, tagged with RFP or Venus YFP, to mark cells in S, G2, and M phases of cell cycle. Reagent of the Fly-FUCCI system. [78]
55106, 55107, 55108, 55109 55115, 55116, 55119, 55120 QUAS-X∷E2F1 E2F1, tagged with CFP or GFP, to mark cells in S, G2, and M phases of cell cycle. Reagent of the Fly-FUCCI system. [78]
30008, 30126, 30127 QUAS-FLP FLP recombinase [15]
52226, 52227 QUAS-R-GECO RGECO calcium reporter [58]
30001, 30002, 30003 QUAS-mCD8:GFP Membrane-bound GFP [15]
52263, 52264 10xQUAS-6XGFP Cytoplasmic hexameric GFP for extra-strong labeling. [44]
36351 QUAS-nSyb:mCherry-HA Cherry- and HA-tagged n-syb protein for labeling presynaptic terminals [74]
36358 QUAS-nSyb:GFP-myc GFP- and myc-tagged n-syb protein for labeling presynaptic terminals [74]
30134, 30135, 30136 QUAS-FRT-stop-FRT-mCD8:GFP GFP, fused to membrane tag CD8, expressed upon FLP-mediated removal of transcription stop [15]
36355, 36366 QUAS-eNpHR Halorhodopsin, inhibitory yellow light-activated chloride channel. [74]
30006, 30007 QUAS-nuclacZ LacZ (beta-galactosidase) for labeling nuclei. [15]
36352, 36353, 36363, 36364 QUAS-Rab3 Synaptic vesicle membrane-associated protein Rab3, tagged with GFP or mCherry. [74, 76]
52255 10xQUAS-2xHA-Rab3 Synaptic vesicle membrane-associated protein Rab3, tagged with HA. [74, 76]
52269, 52270 10xQUAS-6XmCherry Cytoplasmic hexameric red fluorescent protein mCherry for extra-strong labeling. [44]
30004, 30005, 30037, 30043 30118 QUAS-mtdTomato-3xHA Membrane-bound red fluorescent protein tdTomato. [15]
30128 QUAS-FRT-stop-FRT-shibirets Temperature-sensitive shibire protein, expressed upon FLP-mediated recombination that removes the transcriptional stop cassette. [15]
30010, 30011, 30012, 30013 QUAS-shibirets Temperature-sensitive shibire protein. Inhibits synaptic transmission at 29 °C and above. [15]
41565, 41566 QUAS-Ten-a Presynaptic teneurin [51]
41571, 41572 QUAS-Ten-m Postsynaptic teneurin [51]
61658, 61811 QUAS-p65AD∷CaM p65 activation domain, fused to Calmodulin. Component of activity-dependent labeling system TRIC. [61]
61659, 61660 QUAS-FRT-stop-FRT-p65AD∷CaM p65 activation domain, fused to Calmodulin. Expressed upon FLP-mediated recombination that removes the transcriptional stop cassette. Component of activity-dependent labeling system TRIC. [61]
52230 QUAS-ChIEF-tdTomato A variant of Channelrhodopsin, excitatory blue light-activated sodium channel, tagged with tdTomato. Contributed by Kaiyu Wang
30009 QUAS-dm Diminutive (Drosophila myc). Contributed by Christopher Potter and Liqun Luo
51948, 51949, 51950 QUAS-GAL80 GAL80, repressor of GAL4 Contributed by Christopher Potter
52231 QUAS-GCaMP3 GCaMP3 calcium reporter for live imaging Contributed by Kaiyu Wang
QUAS-spH Synapto-pHluorin, a pH-sensitive mutant of GFP that allows to monitor synaptic vesicle release. [57]
QUAS-Brp-Short-mStraw Bruchpilot-Short protein, fused to mStrawberry, to label sites of presynaptic neurotransmitter release. [52]
QUAS-GFP11 A GRASP version of GFP for labeling synaptic connections. [79]
QS repressor lines
30021, 30022, 30024, 52112 αTubulin-QS Expresses QS, the repressor of TA’s with QF AD, under the control of αTubulin84B promoter [15]
30033 UAS-QS Expresses QS, the repressor of TA’s with QF AD, under the control of UAS [15]