Figure 2.

Cholesterol inhibits ATP-induced eNOS phosphorylation. Cells were simulated with 100 µM ATP with Ca⁺². Cells were treated with either PBS with Ca⁺² or 3.5 mM cholesterol at 37 °C for 30 min prior to stimulation. Cells were harvested before stimulation (t = 0) and at time points 1, 3, 5, and 10 min after stimulation. All p-eNOS/eNOS ratios were normalized by t = 0. eNOS phosphorylation in cholesterol enriched cells (Cholesterol) is abolished in response to ATP. (Mean and SEM were plotted p < 0.05*, #; p < 0.01** two-tailed t test; Unenriched n = 4, Cholesterol n = 4).