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Cholesterol impairs the shear stress-induced increase in eNOS phosphorylation. Bar graph representation of the ratio of phosphorylated eNOS to total eNOS for unenriched and cholesterol enriched cells following exposure to 10 dyn/cm2 for 3 min. The response is normalized by the unenriched control (Mean and SEM were plotted, ***p < 0.001 one-tailed t test n = 5 unenriched, n = 6 cholesterol).
