Figure 6.

Inhibition of AKT, β-catenin, and STAT5 signaling blocks 5 GF effects on CD49f⁺ CB cell survival and proliferation. (A) Mean signaling differences from DMSO control in OCI-AML5 following stimulation with 5 GFs, determined after 5 minutes in the presence of triciribine or U0126, and after 15 minutes in the presence of the other inhibitors. (B) Kaplan-Meier survival curves for single CD49f⁺ cells cultured in either 5 GFs ± 1 µM U0126 (a MEK inhibitor), 3.1 µM FR180204 (an ERK1/2 inhibitor), 1.5 µM H89 (a PKA inhibitor), 15 µM STAT5 inhibitor (STAT5i), 50 µM PNU74654 (a β-catenin inhibitor), or 400 nM triciribine (an AKT inhibitor). (C) Percent of viable single cells observed to undergo 1, 2, and 3 cell divisions in the presence of 5 GFs ± the indicated inhibitors. (D) Cumulative distribution functions of the timing of the first, second, and third cell divisions of single CD49f⁺ cells cultured in 5 GFs ± the indicated inhibitors, where the second and third divisions were calculated for each clone individually. Median division times are indicated by the dotted lines. Statistical significance was tested compared with 5 GFs + DMSO. An FDR correction for multiple testing was applied. ^•P = .1; *P = .05; **P = .01; ***P ≤ .001. The legend in the top right indicates the color corresponding to each condition tested.