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. 2017 Jan 27;12(1):e0170213. doi: 10.1371/journal.pone.0170213

Fig 3. Prolonged activation of Htr2b reduces GSIS in MIN6 cells.

Fig 3

(A) MIN6 cells were treated with vehicle or increasing concentrations (10 nM– 10 μM) of the selective Htr2b agonist BW723C86 for 6 hours and then subjected to GSIS procedure. (B) MIN6 cells were treated with vehicle, 50 μM 5HT or co-treated with 5HT (50 μM) plus a selective Htr2b antagonist SB204741 (100 nM) for 6 hours and then subjected to GSIS procedure. The GSIS assays were carry out with KRH buffer 0 (white bars) or 20 mM glucose (black bars). Concentration of insulin accumulated over 1 hour was measured by ELISA. The bars represent mean ± SEM of three independent experiments in triplicate. Number over the bars represents the stimulation index (SI). ** p<0.01, *** p<0.001 and ns, non-significant. One-way ANOVA analysis.