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. 2017 Jan 27;12(1):e0170213. doi: 10.1371/journal.pone.0170213

Fig 4. Prolonged Htr2b activation increases PGC1α and PPARy levels in MIN6 cells and the effect of PGC1α1 overexpression on GSIS.

Fig 4

(A) MIN6 cells were treated for 6 hours with the Htr2b selective agonist BW723C86 (10 μM) and then the mRNA levels of PGC1α and PPARy were quantified by RT-qPCR. Relative mRNA levels are expressed as fold-change of 2−ΔCt in treated vs. control cells (2−ΔCt treated/2−ΔCt control). Numbers over bars correspond to mean Ct values. (B) MIN6 cells were treated for 24 hours with BW723C86 (10 μM) and then, PGC1α and Gapdh protein expression was evaluated by immunoblot. (C and D) MIN6 cells were transfected with a pcDNA3.1-PGC1α1 or an empty vector (mock) for 48 hours. (C) Protein levels of PGC1α1 and (D) insulin secretion were measured by immunoblot and GSIS procedure, respectively. Graph bars represent means ± SEM of three independent experiments in triplicate. ** p<0.01 and *** p<0.001 in one-way ANOVA analysis (A and D) or Student’s t test (B and C).