PCR detection of AAV vector sequences in hearts and several extra-cardiac organs after intramyocardial injection of the vectors. (Upper) The PCR results with heart DNA. N, normal heart; 2 weeks, hearts collected 2 weeks after vector injection; Hepes, Hepes-injected heart; 6 months, hearts collected 6 months after vector injection. (Lower) An example of organs in one of the mice collected 2 weeks after intramyocardial injection. B, brain; H, heart; L, lung; Li, liver; K, kidney; S, spleen; O, ovary; D, diaphragm.