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. 2004 Nov 8;101(46):16280–16285. doi: 10.1073/pnas.0407449101

Fig. 4.

Fig. 4.

VEGF and LacZ expression in livers of mice that have AAV sequences after receiving intramyocardial injection of CMVVEGF/CMVLacZ (columns A) and MLCVEGF/CMVLacZ (columns B and C). Columns A and C, normal heart mice; columns B, ischemic heart mice. The y axis indicates the gene expression (relative value), calculated based on the threshold cycle (CT). Using the expression of mouse HPRT as the basis for comparison, the relative value of VEGF expression was calculated as 2-ΔCTVEGF where ΔCTVEGF = CTVEGF-CTmHPRT, and the relative value of LacZ expression was calculated as 2-ΔCTLacZ where ΔCTLacZ = CTLacZ-CTmHPRT. The relative value of VEGF expression in livers of CMVVEGF-injected normal heart mice was 26,615.9 ± 1.37. VEGF expression was not detectable in livers of MLCVEGF-injected mice with either normal or ischemic hearts. The relative values of LacZ expression in livers were 10,085.5 ± 2.03 (CMVVEGF/CMVLacZ group), 11,585.2 ± 1.53 (MLCVEGF/CMVLacZ group with ischemic hearts), and 9,410.1 ± 1.21 (MLCVEGF/CMVLacZ group with normal hearts).