Changes in histone acetylation during neural progenitor lineage progression. Western blot analysis of neural progenitor extracts from undifferentiated (U) cultures grown in N2 media plus 20 ng/ml FGF-2 or differentiated with 1 μM retinoic acid plus 5 μM forskolin for 4 days (neuron, N), 500 ng/ml IGF-1 for 4 days (oligodendrocyte, O), or 50 ng/ml LIF plus 50 ng/ml BMP-2 for 6 days (astrocyte, A). Immunoblotting was performed by using antibodies against histones H3 and H4 and acetylated histones H3 and H4. Data shown are from at least three independent experiments.