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. 2004 Nov 10;101(47):16659–16664. doi: 10.1073/pnas.0407643101

Fig. 4.

Fig. 4.

VPA up-regulates neuron-specific genes, including the bHLH transcription factor NeuroD.(A) RT-PCR of NeuroD, SCG10, and Synapsin I in neural progenitors at time 0 or treated with 1 mM VPA for 3 h and 24 h. GAPDH was used as a normalization control. (B) Western blot time-course analysis of ERK activation after 1 mM VPA, 100 nM TSA, and 1 mM valpromide (VPM) treatment. (C) ERK activation with 1 mM VPA treatment with and without addition of U0126 (different doses are indicated). (D) RT-PCR analysis of VPA-mediated up-regulation of NeuroD (3 and 24 h) with and without U0126 (1 μM).