Fig 5. Initial Rounds of Replication are Sufficient for Late Gene Expression.

Vero cells were infected with wild type HSV-1 at an MOI of 10 PFU/cell and acyclovir was added to the growth medium as indicated at 0, 2, 3, 4, or 6 hpi. Total DNA or RNA was collected at 12 hpi. A. The number of viral genomes was measured by quantification of the gC gene by qPCR. Error bars represent the standard deviation of biological duplicates. B-E. The quantities of viral transcripts were measured using RNA-Seq. B. RNA-Seq analysis of the late gene UL44 (gC). The units on the y-axis are reads mapped to the viral gene locus per million total reads per kilobase pair (Reads/10⁶ TR/KB). C. RNA-Seq analysis of the early gene UL23 (tk). D. Mapped reads are shown relative to the HSV-1 gene loci from UL39 to UL52. Each sample was normalized relative to 10⁷ total reads. The y-axis maximum value is 75,000 reads. The gray shaded areas indicate late genes. E. Mapped reads are shown relative to the KOS gene loci map from UL22 to UL30. Dashed boxes indicate early genes UL23 (tk) and UL29 (ICP8).