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. 2017 Jan 17;11(1):e0005306. doi: 10.1371/journal.pntd.0005306

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© 2017 Krautz-Peterson et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Total extracts from juvenile or adult mammalian-stage Schistosoma mansoni worms were resolved by SDS-PAGE under non-reducing conditions and transferred to filters. Filters were probed by one of four different anti-tegumental scFvs (Teg1, S3. Teg4 and Teg5, all at 1 μg/ml) previously obtained from S. mansoni infected rats. Detection was by HRP/anti-E-tag recognition of a peptide E-tag expressed with each scFv. Numbers indicate the positions of migration of molecular mass markers (kDa).