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. 2017 Jan 17;11(1):e0005306. doi: 10.1371/journal.pntd.0005306

Fig 4. S. mansoni infected rat sera recognize reduction-sensitive epitopes in recombinant insect cell extracts containing SmTsp2, Sm23 or Sm29.

Fig 4

Insect cells engineered to express selected SmTsp2, Sm23 or Sm29 were extracted in a mild detergent buffer and resolved by SDS-PAGE under non-reducing (-) or reducing (+) conditions. A. Filters containing insect cell extracts containing SmTsp2 were probed with Teg1 scFv or rat infection sera (1:2000). Detection employed HRP/anti-E-tag for Teg1 scFv, or HRP/anti-rat IgG. B. Filters containing insect cell extracts containing Sm23 were probed with S3 scFv or rat infection sera (1:2000). Detection employed HRP/anti-E-tag for S3 scFv, or HRP/anti-rat IgG. C. Filters containing insect cell extracts containing Sm29 were probed with Teg4 scFv or rat infection sera (1:2000). Detection employed HRP/anti-E-tag for Teg4 scFv, or HRP/anti-rat IgG. Numbers indicate the positions of migration of molecular mass markers (kDa).