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. 2017 Jan 17;11(1):e0005306. doi: 10.1371/journal.pntd.0005306

Fig 10. Insect extracts containing recombinant tetraspanins, SmTsp2 and Sm23 block recognition of a major 20 kDa reduction-sensitive S. mansoni antigen on western blots by mouse, rat and human infection sera.

Fig 10

Total extracts from juvenile or adult mammalian-stage Schistosoma mansoni worms were resolved by SDS-PAGE under non-reducing conditions and transferred to filters. Pooled mouse, rat or human infection sera (1:2000) were pre-incubated for 1 hr with control lysate buffer or lysates of insect cells expressing SmTsp2 or Sm23 (as indicated) prior to overnight incubation with the filter strips. Detection employed the appropriate HRP/anti-mouse, -rat or -human IgG reagent. The arrow indicates the position of migration of SmTsp2 and Sm23. Numbers indicate the positions of migration of molecular mass markers (kDa).