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. 2017 Jan 17;11(1):e0005306. doi: 10.1371/journal.pntd.0005306

Fig 11. S. mansoni infected rat sera recognition of SmLy6C, SmTsp2 and Sm29 can be substantially suppressed by a single monoclonal scFv antibody.

Fig 11

Detergent extracts of recombinant insect cells (A), or total extracts from juvenile or adult mammalian-stage Schistosoma mansoni worms (B) were resolved by SDS-PAGE under non-reducing conditions and transferred to filters. Replicate filter strips containing the indicated extracts were pre-incubated with the indicated scFv (Teg1, Teg4, Teg5 or S3, all at 10 μg/ml) or with buffer alone for 1 hr with S. mansoni rat infection sera (1:2000) prior to overnight incubation with the filter strips. Detection employed HRP/anti-rat IgG. Arrow indicates the position of migration of Sm29 and SmTsp2.Sm23. Numbers indicate the positions of migration of molecular mass markers (kDa).