. 2004 Nov;136(3):3504–3514. doi: 10.1104/pp.104.049718

Table II.

Primers and probes used in TaqMan real-time quantitative RT-PCR assay

LeCCD1A	Forward	TTGATTACCTGCCGCCTTGT
	Reverse	CATATAGCTCATTGCAGAAATTC
	Probe^a	AACCCAGACCTAGACATGGTCAATGGAGC
PhCCD1	Forward	TCATATTTCACAACGCCAATGC
	Reverse	CGGAAGGCGGCAGGTAA
	Probe^a	TGGGAGGAGGGAGATGAAGTCGTGTTG

Primers and probes were designed using PRIMER EXPRESS software (Applied Biosystems).

All probes were labeled at the 5′ with fluorescent reporter dye 6-carboxyfluorescein and at the 3′ with black hole quencher-1 from Integrated DNA Technologies (Coralville, IA).