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Distribution of Ca2+ in the pollen grain during in vitro germination. a, A ratiometric image of the pollen grain after hydration. b, A ratiometric image of a protrusion on a pollen grain. [Ca2+]cyt increase was observed not only at the potential germination site (arrow) but also at another site (arrowhead). c, A ratiometric image of a nifedipine-treated pollen grain after hydration.
