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. 2004 Nov;136(3):3572–3581. doi: 10.1104/pp.104.045195

Figure 1.

Figure 1.

Evidence of the progressive loss of nuclei in the P-C layers of Mn1 and mn1 maize caryopses. A, DAPI-stained median longitudinal sections of the basal part of caryopses at 0 to 10 DAP; arrows in 4 DAP point to the first cells without visible nuclei. BI, A schematic drawing of the basal part of maize caryopsis at about 10 DAP. BII, Basal endosperm and pedicel can be subdivided into several zones: (1) endosperm; (2) BETC; (3) nucellar P-C layer; (4) closing layer; (4 and 5) integumental P-C layer; and (6) pedicel. BIII, Endosperm and the various layers at the base of the caryopsis that give rise to the P-C layers: (1) endosperm; (2) BETC; (3) nucellus epidermis; (4) semipermeable membrane; (5) outer integument; (6) inner pericarp; and (7) mid-pericarp. C, DAPI-stained 12-DAP fertilized caryopsis and unfertilized ovule from the same ear. em, embryo; en, endosperm; es, embryo sac; n, nucellus; p, pericarp; pc, P-C layer; ped, pedicel; v, vascular tissue.