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. Author manuscript; available in PMC: 2018 Jan 15.
Published in final edited form as: Virology. 2016 Nov 30;501:119–126. doi: 10.1016/j.virol.2016.11.016

Figure 3.

Figure 3

vFLIP and RTA interact with endogenous Itch. A. RTA interacts with endogenous Itch and vFLIP. 293T cells were transfected with vFLIP and RTA. 24h post-transfection, cells were treated with 1uM MG132. 48h post transfection, cells were harvested and processed for immunoprecipitation. Precleared lysates were incubated with antibodies against RTA followed by addition of protein A/G agarose and washed with RIPA buffer. Immunoprecipitates were analyzed by SDS-PAGE followed by immunoblot using antibodies against myc, RTA, and Itch. B. vFLIP interacts with endogenous Itch. 293T cells were transfected with vFLIP and RTA. 24h post-transfection, cells were treated with 1uM MG132. 48h post transfection, cells were harvested and processed for immunoprecipitation. Precleared lysates were incubated with antibodies against myc-vFLIP followed by addition of protein A/G agarose and washed with RIPA buffer. Immunoprecipitates were analyzed by SDS-PAGE followed by immunoblot using antibodies against myc and Itch. *Asterisk denotes light chain (LC) band.