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. Author manuscript; available in PMC: 2018 Jan 15.
Published in final edited form as: Virology. 2016 Nov 30;501:119–126. doi: 10.1016/j.virol.2016.11.016

Figure 4.

Figure 4

RTA interacts with Itch and induces the degradation of vFLIP in naturally infected TREx BCBL1-RTA cells. A. RTA interacts with Itch. TREx BCBL1-RTA cells were treated with doxycycline (1ug/ml) for 0 or 24h to induce RTA expression. Cells were harvested and processed for immunoprecipitation with antibodies against RTA. Immunoprecipitates were analyzed by SDS PAGE followed by immunoblot against RTA and endogenous Itch. B. RTA induces the degradation of vFLIP in naturally infected cells. TREx BCBL1-RTA cells were treated with doxycycline (1ug/ml) for 0, 24 or 48h to induce RTA expression. Cells were harvested and analyzed by SDS PAGE followed by immunoblot against vFLIP, RTA and endogenous beta actin.