(A) Conceptual illustration of carbon incorporation and transfer between trophic levels for an autotrophic (NPlow and NPhigh)–based and heterotrophic (TM)–based pelagic food web, illustrating an increased number of trophic levels as a potential cause of enhanced MeHg bioaccumulation in a heterotrophic food web. DIC, dissolved inorganic carbon. (B and C) Average MeHg concentration (pmol g−1 d.w.) in seston size fractions (50 to 100, 100 to 300, and >300 μm; n = 3 for each fraction) and average for all size fractions (n = 9) collected at the end of the experiment (day 57) from the different mesocosm treatments (n = 3 per treatment), that is, low nutrient input (NPlow, blue bars), high nutrient input (NPhigh, green bars), and terrestrial NOM input (TM, red bars). Error bars are given as SE. (B) Bioaccumulation of MeHg originating from the HgII tracer added into the water (204HgIIwt) and (C) bioaccumulation of MeHg originating from the MeHg tracer added to the water column (Me199Hgwt).