Characterization of dark-grown Atnap-1 and Atpir-1 mutant phenotypes. A to C, Fifteen-day-old dark-grown seedlings of Col wild type (A), Atnap-1 (B), and Atpir-1 (C) show partially developed cotyledonary petioles and expansion of the cotyledon in wild type (A), and the fully developed cotyledonary petioles, cotyledons, and the first pair of true leaves in Atnap-1 (B) and Atpir-1 (C). The scale bars represent 2 mm in A to C. D, Germination of wild-type, Atnap-1, and Atpir-1 seedlings. Seeds were stratified for 6 d on Murashige and Skoog medium supplemented with 1% Glc. Germination was scored as the percentage of seeds showing radicle emergence at 20°C in constant light. E, Hypocotyl length of 15-d-old dark grown seedlings of wild type, Atnap-1, and Atpir-1 grown on vertical plates. Error bars represent sds (n > 50). F, Hypocotyl diameter of 15-d-old dark-grown seedlings of wild type, Atnap-1, and Atpir-1 grown on vertical plates. Error bars represent sds (n > 50). G, Hypocotyl length of 7-d-old dark-grown seedlings of wild type, Atnap-1, and Atpir-1 grown on vertical plates at different Glc concentrations. Error bars represent sds (n > 30). H, Root length of dark-grown seedlings of wild-type, Atnap-1, and Atpir-1 seedlings grown on vertical plates. Error bars represent sds (n > 50).