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. 2017 Jan 6;6:e21843. doi: 10.7554/eLife.21843

Figure 1. Optogenetic vM1 activation and passive ramp and hold vibrissa deflection.

(A) Scheme of the experimental design with the recording electrode in vS1, optogenetic stimulation of vM1 and ramp and hold passive vibrissa deflection. (B) Peri-stimulus histograms (PSTH, mean ± SEM) recorded from a vS1 neuron during isolated vM1 optogenetic stimulation (blue), isolated passive vibrissa deflection (green), and paired vM1-vibrissa stimulation (red). In addition, the expected linear sum of the solitary vM1 stimulation and vibrissa deflection is shown (gray). (C) Fluorescence images at different magnifications of neurons co-expressing ChR2 and mCherry. (D) PSTH recorded from a vM1 neuron during optogenetic stimulation (473 nm laser pulse). (E) Average (mean ± SEM) responses recorded in vS1 neurons during optogenetic stimulation of vM1 with laser pulse duration (147 neurons in four rats). In each neurons, the spike count responses during optohgenetic stimulation were presented as percent of the spike count during the pre-stimulus control value. Later, the responses were averaged over the different neurons.

DOI: http://dx.doi.org/10.7554/eLife.21843.002

Figure 1.

Figure 1—figure supplement 1. Raw data of unit recordings.

Figure 1—figure supplement 1.

Four separate traces of raw recording filtered at 1–5 kHz. Each trace shows simultaneous recordings from eight different channels.
DOI: http://dx.doi.org/10.7554/eLife.21843.003
Figure 1—figure supplement 2. Raw clustering data.

Figure 1—figure supplement 2.

The raw clustering data is presented for ten individual electrodes, which yielded 26 individual sorted units. For each electrode, we present the putative layer, the 2D principal components graph, the mean ± SD of each unit and the clustering statistical parameters.
DOI: http://dx.doi.org/10.7554/eLife.21843.004
Figure 1—figure supplement 3. Piezo bimorph movement.

Figure 1—figure supplement 3.

The movement of the piezo bimorph was monitored during a ramp and hold stimulation pulse using a high-speed camera (1000 fps). Panel A shows individual traces of piezo bimorph movement after applying different values to the smoothening factor (0.03–1). Panel B presents the average amplitude of the maximal ringing (measured from peak to turf) with the different smoothening factor values.
DOI: http://dx.doi.org/10.7554/eLife.21843.005