Fig 3. Srp mRNAs are stabilized following CELF1 knockdown.

A: Control (white bars) and CELF1 KD (filled bars) C2C12 cells were treated with 4sU and total RNA was isolated at 12 hours. Following biotinylation and fractionation, the abundance of each Srp mRNA in total and labeled fractions was determined by dPCR and used to derive the half-life. The results were derived from three independent experiments for all except Srp54 and 7SL RNAs which were derived from two experiments. The error bars represent the standard error of the mean. Asterisks denote p<0.05. The inset shows a western blot verifying that CELF1 protein was effectively undetectable in CELF1 KD cells, GAPDH was evaluated as a loading control. B: Srp mRNA half-lives in Control and CELF1 KD C2C12 cells derived from the graphs in (A) are plotted for comparison.