Skip to main content
. 2017 Jan 27;12(1):e0170680. doi: 10.1371/journal.pone.0170680

Fig 5. CELF1 KD cells have increased secretory capacity and perform poorly in wound healing assays.

Fig 5

A: Control (Ctrl) and CELF1 KD cells were co-transfected with constructs encoding secreted and cytoplasmic luciferase proteins. Gaussia luciferase activity in the media was normalized to cytoplasmic firefly luciferase activity. Errors represent the standard deviation from three independent experiments. B: Control (Ctrl) and CELF1 KD cells were grown to ~90% confluence and then displaced by scratching. The cells were imaged immediately and again after incubation. C: The number of cells migrating into the wound is represented as a percentage of the number in the control cells. The error bars represent the standard deviation from three experiments. D: Western blot demonstrating that SRP68 is over-expressed following transfection of a construct encoding the murine SRP68 protein. E: Following transfection of the SRP68 over-expression construct or a control empty vector, wound healing assays were performed as described for B. F: Wound healing was quantified as for C.