Table 1. CELF1 binding to 3’UTR of Srp mRNAs in vitro.
| Gene | 3’UTR length (nt) | Putative CELF1 binding sites | Kd (nM) | ||
|---|---|---|---|---|---|
| Srp9 | 960 | GUGUUUUUUGUA | GUGUUGUC | 0.84 ± 0.12 | |
| Srp14 | 380 | UUGUGUU | 12.4 ± 1.8 | ||
| Srp19 | 371 | UUUGUUUGUC | 33.8 ± 1.7 | ||
| Srp54 | 2475 | UUCUGUGUUCUUGU | AGGUGUUUUUCUG | 13.5 ± 1.0 | |
| Srp68 | 536 | UUGUGUGUUUGUG | GUGUUUGUC | CUGUGUC | 1.64 ± 0.29 |
| Srp72 | 1566 | GUGUGUGUGUAUUUGUG | UGUACCUUUGUUGUUUC | 0.53 ± 0.08 | |
GU-rich regions contained within each fragment that could act as CELF1 binding sites are shown. 3’UTR fragments ranging from 120–264 nt in length containing GU-rich elements were used for electrophoretic mobility shifts to derive the dissociation constants. Errors are standard deviations from three independent replicates.