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. 2004 Nov;136(3):3784–3794. doi: 10.1104/pp.104.046656

Figure 1.

Figure 1.

Expression of PMSR4 in wild-type plants in response to oxidative stress. A, Western blot using anti-PMSR4 antibody. B, Enzyme activity of the soluble fraction of three independent preparations of isolated chloroplasts from 32 plants as described in “Materials and Methods.” Control (CON), Control plants without treatment. High light (HL), Plants exposed to 600 μmol photons m−2 s−1 at 8°C for 48 h. Paraquat (PQ), Plants sprayed with 10 μm methyl viologen (paraquat). Ozone (O3), Plants exposed to 0.46 μL L−1 O3 for 6 h during 4 d. PMSR4 activity results show the mean and sd (values above bars). Means with the same letter above the values are not statistically different (P ≤ 0.05). Purity of the chloroplast preparation was assessed by AAT activity gels. C, AAT activity gel. Total crude extracts (T) exhibit two activity bands corresponding to cytosol (cytosolic) and chloroplast (chloroplastic) isozymes. Chloroplast preparations (C) show only one band. D, Loading control for western blot shown in A. The nitrocellulose membrane was stained with Red Punceau.